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KMID : 0605820100170020074
Korean Journal of Pediatric Infectious Diseases
2010 Volume.17 No. 2 p.74 ~ p.82
Evaluation of Uricult Trio Test as a Rapid Screening of UTI in Children with Fever
Lee Jin

Kim Eun-Jin
Lee Teak-Jin
Chang Jin-Keun
Cha Sung-Ho
Abstract
Purpose : The Dipslide culture test is a rapid method for diagnosis of urinary tract infection (UTI). The aim of this study is to determine the diagnostic availability of a urine Dipslide test for evaluation of UTI in febrile children.

Methods : Urine specimens from 151 febrile infants were inoculated by a routine blood agar urine culture method and the Dipslide test at the same time. Following incubation for 16-24 hours, the results of the Dipslide test were read at the next visit. Both results of Dipslide and those of routine culture were compared.

Results : The mean age of subjects was 15¡¾10.6 months. There were 150 infants (99.3%) who had fever with a mean duration of 2.6¡¾2.6 days. Thirty two infants (21.2%) were diagnosed as having UTI. Sensitivity and specificity of Uricult Trio CLED medium were 59.4% and 84.8%, respectively. Sensitivity and specificity of Uricult Trio E. coli medium were 60.0% and 96.2 %, respectively. The Pearson correlation coefficient between results of Uricult Trio CLED medium and urine culture was 0.438 (P =0.01). Correlation between results of Uricult Trio E. coli medium and urine culture was 0.617 (P =0.01).

Conclusion : The Dipslide test requires only 16-24 hours with high specificity in terms of UTI caused by E. coli without the problems associated with specimen delay. This test seems to be helpful for exclusion of UTI in febrile infants and it may reduce unnecessary hospitalization and antibiotic use. However, further studies are required before the product can be recommended as a routine diagnostic tool.
KEYWORD
Urinary tract infection, Dipslide, Uricult Trio, Children
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